|Posted by mcoggins on July 16, 2018 at 10:55 AM|
With new cutting edge pharmaceuticals relying heavily on recombinant proteins there has been a big push by bioprocessing companies to optimise and increase production of recombinant proteins. These proteins are typically produced in bacterial cells such as e-coli and for more complex proteins mamalian cell lines such as CHO.
However growing proteins in mamalian cells like CHO is challenging as they require optimal enviromental conditions to even produce a small amount of the desired protein. This is why recently the industry has looke towards genetic engineering to alter cell functionality in order to make these cells overproduce the target protein of interest. However altering a cells genome can be expensive and challenging requriing utting edge laboratories.
Our genetic engineering program Snapshot which models the genes and reactions within cells could be a powerful tool for companies that want to edit a cells genome to produce proteins of interest. This is because snaptshot has built in functions that highlight genes associated with reactions that produce metabolites in different cellular compartments.
For example if you wanted to find the genes associated with reactions that produce ATP then you would enter max:atp_c (where c denotes the cytoplasm compartment). This would then highlight all genes associated with producing ATP. Likewise if you wanted to minimise production of a certain metabolite that was deemed toxic or redundant then you would enter min:. For example to minimise lactic_acid which is deemed a waste product you would enter min:l_lactic_acid_c.
Screenshot above showing snapshot highlighting HGNC:9021 and 9020 associated with producing ATP